A Practical Book of Systemic Pharmacology II

About This Book

This practical book has been carefully prepared to complement the theoretical knowledge acquired by students of Bachelor of Pharmacy (B.Pharm) V Semester. It aims to bridge the gap between classroom learning and practical application by providing structured experiments, relevant pharmacological exercises, and opportunities to develop analytical and interpretative skills. The contents have been organized to facilitate systematic learning and to strengthen students' understanding of drug actions, therapeutic responses, and the principles underlying pharmacological investigations.

Special emphasis has been placed on clarity, conceptual understanding, and a student-friendly presentation. The practical exercises encourage scientific observation, logical reasoning, accurate recording of results, and meaningful interpretation of experimental findings. Appropriate attention to laboratory safety, ethical considerations, and the responsible use of experimental methods runs throughout; where applicable, simulations, demonstrations and other appropriate alternatives are used to support learning while respecting ethical principles.

Prepared with reference to the new Pharmacy Council of India (PCI) Syllabus 2026 and the vision of the National Education Policy (NEP) 2020, this book promotes outcome-based learning and the development of the practical competencies required in pharmaceutical sciences. It is intended to serve as a useful resource for undergraduate pharmacy students and a supportive guide for teachers conducting pharmacology practical sessions.

List of Experiments

1. Evaluation of analgesic activity by hot plate, tail-flick, tail immersion and acetic acid-induced writhing methods
2. Evaluation of antiepileptic activity using maximal electroconvulsive shock (MES)-induced seizures
3. Demonstration of antiepileptic activity using pentylenetetrazol-induced seizures
4. Evaluation of antidepressant activity of drugs using the tail suspension test
5. Demonstration and study of locomotor activity using actophotometer
6. Evaluation of antianxiety activity using the elevated plus maze or zero maze model
7. Evaluation of antipsychotic activity using inhibition of conditioned response on Cook's pole climbing apparatus
8. Study of the effect of drugs on learning and memory using the Morris water maze test
9. Study of antiulcer activity using indomethacin-induced or pylorus ligation-induced ulcer models
10. Estimation of the concentration of oxytocin on isolated rat uterus preparation
11. Estimation of drug concentration by 3-point or 4-point bioassay
12. Bioassay of histamine using matching, bracketing or interpolation methods on isolated tissue
13. Study of cell culture techniques: types of cell culture, instruments, culture media and growth of cell cultures
14. Study of antibacterial sensitivity testing of urine culture by the disc diffusion method
15. Demonstration using databases and software packages for predicting drug activity, ADME properties and toxicity
16. Study of electrophoresis of protein and DNA samples and gel visualization techniques
17. Construction of pharmacological networks using predicted drug targets and disease genes

Keywords

How to Cite

Girotra, P., & Rao, P. (2026). A Practical Book of Systemic Pharmacology II (1st ed.). WiseLeaf Scientific Ventures. ISBN: 978-81-181068-9-0