Systemic Pharmacology II (Practical)

About This Book

The practical exercises cover important areas of systemic pharmacology, presenting screening models for analgesic, antiepileptic, antidepressant, antianxiety, antipsychotic and antiulcer activity, studies on learning and memory, bioassay of oxytocin and histamine on isolated tissue preparations, cell culture techniques and antibacterial sensitivity testing, each set out in a clear and structured manner.

In keeping with contemporary trends in pharmacological education and the principles of the 3Rs (Replacement, Reduction and Refinement), appropriate emphasis has been given to computer-based simulations and alternative experimental approaches. Students are introduced to simulation models, dose–response relationships, bioassay concepts, antagonist studies and the interpretation of pharmacological data, along with modern approaches such as in silico pharmacology, ADME and toxicity prediction, pharmacological databases and network pharmacology. Each practical has been developed to encourage conceptual understanding rather than mere procedural learning.

List of Experiments

1. Evaluation of Analgesic Activity by Eddy's Hot Plate, Tail Flick, Tail Immersion and Acetic Acid Writhing
2. Evaluation of Antiepileptic Activity by Maximal Electroshock-Induced Seizures
3. Demonstration of Antiepileptic Activity Using Pentylenetetrazol-Induced Seizures
4. Evaluation of Antidepressant Activity by the Tail Suspension Test
5. Demonstration and Study of Locomotor Activity Using the Actophotometer
6. Evaluation of Antianxiety Activity Using the Elevated Plus Maze and the Elevated Zero Maze
7. Evaluation of Antipsychotic Activity by Inhibition of the Conditioned Avoidance Response on Cook's Pole Climbing Apparatus
8. Study of the Effect of Drugs on Learning and Memory Using the Morris Water Maze
9. Study of Antiulcer Activity Using the Indomethacin-Induced and Pylorus Ligation-Induced Ulcer Models
10. Estimation of the Concentration of Oxytocin on the Isolated Rat Uterus Preparation
11. Estimation of Drug Concentration by Three-Point and Four-Point Bioassay
12. Bioassay of Histamine by Matching, Bracketing and Interpolation on the Isolated Guinea Pig Ileum
13. Study of Cell Culture Techniques
14. Antibacterial Sensitivity Testing of Urine Culture by the Disc Diffusion Method
15. Demonstration of Databases and Software for Predicting Drug Activity, ADME Properties and Toxicity
16. Electrophoresis of Protein and DNA Samples and Gel Visualisation Techniques
17. Construction of Pharmacological Networks Using Predicted Drug Targets and Disease Genes

Keywords

How to Cite

Saklani, S., Khan, S., Bhandari, A., & Bisht, T. (2026). Systemic Pharmacology II (Practical) (1st ed.). WiseLeaf Scientific Ventures. ISBN: 978-81-180383-3-7